POSTNATAL DEVELOPMENT OF EXPRESSION OF mGLUR2/3 AND GLUR2/3 IN THE PRIMARY VISUAL CORTEX OF THE MACAQUE MONKEY.
Society for Neuroscience 1998
We examined the postnatal developmental pattern of expression of metabotropic (mGluR2/3) and AMPA-type(GluR2/3) glutamate receptors in the striate cortex (V1) of Japanese monkeys (Macaca fuscata) using immunohistochemical methods. An antibody against mGluR2/3 stained neuropils, and the laminar stainig pattern changed obviously with age. In 2- to 5-week-old monkeys (n=2), all layers of the primary visual cortex were immunoreactive, with more pronounced staining in layers 4A, 4B, 4Cƒ¿ and 5A. In 2- to 8-month-old monkeys (n=5), staining of layers 4Cƒ¿ and 5B was weak compared to the other layers, giving an appearance of two light bands in the middle and lower layers of the cortex. In adults (>4 years old, n=6), the overall staining in V1 was much weaker than in the prestriate cortices (areas V2 and V4) and the inferior temporal cortex (areas TEO and TE). This was in contrast to the even staining noted across all cortical areas in 2-week to 8-month-old monkeys. The laminar difference in adult V1 was not evident due to the weak staining, although layer 2/3 was slightly better stained than the other layers. An antibody against GluR2/3 stained mainly the pyramidal cell bodies of layers 2/3 and 6, and multipolar cells of layer 4B in V1. This laminar distribution pattern did not change during the postnatal period examined (2 weeks to >4 years old). However, the patch-like clusters of strongly GluR2/3-immunoreactive pyramidal cells in layer 2/3, which have previously been shown to be in register with cytochrome oxidase blobs, were observed only after 2 months of age. For all the periods examined, GluR2/3 immunoreactivity in V1 was less pronounced than in the prestriate and inferior temporal cortices.
(Supported by CREST)