Nemoto, S., Fujita, I., Shimizu, N., Nishino, H. and Oomura, H.
Natl. Inst. Physiol. Sci., Okazaki 444
Dept. Physiol., Fac. Med., Kyushu Univ., Fukuoka 812
A series of experiments in our laboratory have shown that 2-deoxytetronic acid lactone (2-DTA: 3, 4-dihydroxybutanoic acid lactone) suppresses food intake and that 3-deoxypentonic acid lactone (3-DPA: 2,4,5-trihydroxypentanoic acid lactone) facilitates food intake. In the present study, we measured plasma concentration of 2-DTA and 3-DPA by high resolution gas chromatography in the rat. Chronic catheter was implanted into the jugular vein of male Wistar rats (body weight: 350-400 g). Blood samples of 300 Ê1 were collected from each rat every l2 hours for 3 days. A half of rats were deprived of foods and the other half had normal diet. In normal feeding rats, the mean concentration of 2-DTA during light time was 110 ÊM, while that of 3-DPA was 260 ÊM. After 48 hours of deprivation, some of food-deprived rats showed increased concentration of 2-DTA which was on average 80 % greater than that of normal feeding rats.