第32回日本神経科学大会

会期:2009.9.16-18
会場: 名古屋国際会議場

マウス一次視覚野の受容野構造地図 : 2光子カルシウムイメージングを用いた逆相関法
Mapping receptive fields on mouse primary visual cortex: reverse correlation using two-photon calcium imaging signals
演題番号 : P2-h12 ポスター発表 発表日:2009.9.17

森 理也(Yoshiya Mori):1 池添 貢司(Koji Ikezoe):1 古高 潤一(Junichi Furutaka):1 喜多村 和郎(Kazuo Kitamura):2 田村 弘(Hiroshi Tamura):1 藤田 一郎(Ichiro Fujita):1 

1:阪大院・生命機能・生命機能(Grad. Sch. of Frontier Biosci., Osaka Univ., Osaka) 2:東京大院・医・医学(Grad. Sch. Med., Univ. of Tokyo, Tokyo)


--------------------------------------------------------------------------------

Visual information is first received by retinal photoreceptors and transmitted to the primary visual cortical (V1) where the layout of visual scene is preserved in a 2D topographic map. We here investigated this retinotopic organization in mouse V1 at the single-neuron spatial resolution by applying two-photon calcium imaging techniques. We determined position and structure of receptive fields (RFs) of a large number of neurons over the cortex by reverse correlating their calcium signals with visual stimuli. RFs thus determined exhibited either Gabor-shaped or center-surround structure. Nearby neuron's RFs were highly overlapped with each other, and their center positions smoothly changed along the tangential direction of the cortex. By contrast, preferred stimulus orientation differed between neighboring neurons. The present results with the single-neuron resolution support the notion that V1 has uniform coverage of stimulus position and orientation.