第27回日本神経科学大会(大阪)

OJ3-12 ER81 is expressed in a subpopulation of layer 5 projection neurons in rodent and primate cerebral cortices

Hiroyuki Yoneshima1, Shinsuke Yamazaki1, Courtney C J Voelker2, Zoltan Molnar2, Ichiro Fujita1, Nobuhiko Yamamoto1

1.Osaka Univ. Grad. School of Frontier Biosci., Osaka, Japan, 2.Dept. of Human Anatomy and Genetics, Univ. of Oxford, Oxford, UK

Lamina-specific gene expression is a key issue to understand cortical cell differentiation. By screening cDNA libraries for the genes enriched in layer 5, we found that ER81, an Ets transcriptional factor, was expressed in a subset of layer 5 neurons throughout the cerebral cortex in mice and rats from embryonic days to young adult stages. ER81 was also detected in layer 5 neurons in infant macaques, but undetectable in adults. Retrograde labeling combined with immunohistochemistry showed that nearly all neurons projecting to the spinal cord and the superior colliculus expressed ER81, while only one-third of the neurons projecting to the contralateral cortical areas expressed the molecule. These findings suggest that ER81 is involved in cellular differentiation of a subset of layer 5 neurons and that this mechanism is preserved among mammalian species.

2004 Elsevier Science Ireland Ltd. and the Japan Neuroscience Society. All rights reserved.